![Buffer cut off wavelength for circular dichroism](https://kumkoniak.com/102.jpg)
For most organic solvents the cut-off is between 210 and 250 nm. For example, using a 0.1M sodium phosphate buffer the wavelength cut-off will be at 195 nm using a 1 cm light path.
![buffer cut off wavelength for circular dichroism buffer cut off wavelength for circular dichroism](https://media.springernature.com/lw685/springer-static/image/art%3A10.1038%2Fs41598-019-39185-3/MediaObjects/41598_2019_39185_Fig1_HTML.png)
The potential of mean force for protein-protein interactions is key to the development of a statistical-mechanical model for salt-induced protein precipitation and crystallization, and for understanding certain disease states, including cataract formation and is the relaxation time of the bulk liquid, it was concluded that more » the more acidic TFE has a weaker hydrogen bond interaction with silica, due to the fact that the alcohols serve as hydrogen bond acceptors. Conformational changes of bovine -lactalbumin induced by adsorption on a hydrophobic interface are studied by fluorescence and circular dichroism. With such a light path, the absorption of the solvent or buffer used also limits the theoretical minimum wavelength (called the cut-off wavelength) you can reach.
![Buffer cut off wavelength for circular dichroism](https://kumkoniak.com/102.jpg)